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10 iu superscript ii reverse transcriptase enzyme  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 10 iu superscript ii reverse transcriptase enzyme
    10 Iu Superscript Ii Reverse Transcriptase Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/10+iu+superscript+ii+reverse+transcriptase+enzyme/pm37635977-277-30-36
    Average 90 stars, based on 1 article reviews
    10 iu superscript ii reverse transcriptase enzyme - by Bioz Stars, 2026-09
    90/100 stars

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    Reverse Transcription:

    Article Title: Neurohypophysial and paracrine vasopressinergic signaling regulates aquaporin trafficking to hydrate marine teleost oocytes.
    Article Snippet: For RT-PCR, total RNA was extracted from different adult tissues, including pituitary, kidney, ovary and isolated ovarian follicles, using the RNeasy Minikit (Qiagen) and DNAse I treatment, following the manufacturer’s instructions. .. Total RNA (5 mg) was reverse transcribed Frontiers in Endocrinology 15 using 0.5 mg oligo(dT)17 primer, 1 mM deoxynucleotide triphosphates (dNTPs), 40 IU RNAse out (Life Technologies Corp.), and 10 IU SuperScript II Reverse Transcriptase enzyme (Life Technologies Corp.) for 1.5 h at 42°C. .. The PCR was carried out with 1 ml of the RT reaction in a final volume of 50 ml containing PCR buffer with Mg2+, 0.2 mM dNTPs, 1 IU of Taq polymerase (Roche), and 0.2 mM of forward and reverse oligonucleotide primers specific for seabream pro-avp, pro-oxt, avpr1aa, avpr2aa and oxtrb (Supplementary Table S1).



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    Thermo Fisher 10 iu superscript ii reverse transcriptase enzyme
    10 Iu Superscript Ii Reverse Transcriptase Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/10+iu+superscript+ii+reverse+transcriptase+enzyme/pm37635977-277-30-36
    Average 90 stars, based on 1 article reviews
    10 iu superscript ii reverse transcriptase enzyme - by Bioz Stars, 2026-09
    90/100 stars
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